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Fig. 4 | Molecular Cancer

Fig. 4

From: MED12 exerts an emerging role in actin-mediated cytokinesis via LIMK2/cofilin pathway in NSCLC

Fig. 4

Loss of MED12 induced multinucleation through cytokinesis defects. a MED12 KO1 of PC9 cells stably expressing either Cherry-vector (red) or GFP-vector (green) were co-cultured. Representative micrographs were shown. Arrows indicated the multinucleate cells. Scale bar: 100 μm. b Live-cell images displayed the division process of WT and MED12 KO of PC9 cells. Black arrowheads indicated intercellular bridge. MED12 WT cell completed cytokinesis successfully in 2.5 h. The cytokinesis of KO1 cells were delayed and failed. Red arrowheads highlighted the cell which attempted to undergo cytokinesis. The two daughter cells remained arrested at the post midbody stage for about 7 h and failed to pass abscission and eventually rejoined one cell again with two nuclei. Scale bar: 25 μm. c Multinucleated cell didn’t pass to the next mitosis for 28 h and crashed eventually. Arrowheads indicated the multinucleated cell. d Immunofluorescence staining for nuclear atypia within MED12 KO cells during cytokinesis with alpha-tubulin and DAPI. Arrowheads denoted heteromorphic nucleus. Scale bar: 10 μm

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