Fig. 2
Conditional TGIF1 deletion accelerates progression to PDAC in cooperation with KrasG12D. a i, Gross morphology of PDAC in 20-week-old Pdx-1Cre KrasG12DTGIF1L/L (PKT) mice. ii, Representative H&E staining images of tissue paraffin sections showing well-differentiated PDAC in PKT mice. Iii, High-power images revealing abnormal anaplastic nuclear features in abundance. b Quantitation of histopathological alterations in the indicated phenotypes of pathology ***p < 0.001. c Kaplan–Meier curves showing the percentage of survival rates of the indicated genotypes (P < 0.01). d PDAC tissue sections of the indicated genotype were stained with Alcian blue and elastic tissue-Masson stain; IHC analysis revealed staining for the proliferating marker Ki67; epithelial markers, CK19 and E-cadherin (E-cad), TGFβ1/p-Smad2, kinase pathway proteins, p-Akt, p-STAT3 and pErk (p-p44/42)and stromal markers, SMA (insert 400 x). Scale bar, 100 μm