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Fig. 4 | Molecular Cancer

Fig. 4

From: Loss of the transcriptional repressor TGIF1 results in enhanced Kras-driven development of pancreatic cancer

Fig. 4

Modulation of the inflammatory cytokine profile and tumor immune response in the PDAC microenvironment by TGIF1. a i, Mouse cytokine arrays profile the cytokine expression levels in PDAC of the PKP and PKTP mice. ii, Template alignment of the mouse cytokines in the array represent: POS, positive; NEG, negative; IL, interleukin; C-C motif chemokine ligand (CCL); SDF-1, stromal cell-derived factor 1; BLC, B-lymphocyte chemo- attractant; TAC, protachykinin; TCA-3, small inducible cytokine A1; TIMP, tissue inhibitors of metalloproteinase; LIX, LPS induced CXC chemokine; MCSF, macrophage colony stimulating factor; MCP-1, monocyte chemotactic protein 1; MIG, mitogen-inducible gene; MIP-1, macrophage inflammatory protein 1. b IHC staining of pancreata from 7-week-old PKP and PPKTP mice with antibodies to detect CD45, CD8, PD-L1, Foxp3 and CD86 (macrophages), CD68, CD163 and arginase (insert 400 x). Scale bar, 100 μm. c Detection of the T-lymphatic marker CD8 and M2-macrophage marker CD68; CD163 expression in the PKP and PKTP PDAC tissues as determined by flow cytometry. The data represent three different experiments. d Western blot analysis for tumor lysate demonstrated increased expression of PD-L1, CD68, CD163 and TGFβ1 in PDAC of the PKTP mice, compared with that in PDAC of PKP mice

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