Fig. 2
From: miR-301a promotes lung tumorigenesis by suppressing Runx3
Deletion of miR-301a alters tumor microenvironment. (a) Representative hematoxylin and eosin-stained histological sections of lungs isolated from KrasLA2 (n = 5) and miR-301a−/−;KrasLA2 (n = 5) mice at 9 weeks of age. Lines highlighted regions of inflammation. Average of tumor nodules were counted on the lung surface of KrasLA2 and miR-301a−/−;KrasLA2 littermates at 9 weeks of age. Quantitative immunohistochemical analysis of leukocytes (b: CD45), T cells (c: CD3), CD4+ T cells (d: CD4), CD8+ T cells (e: CD8), and macrophages (f: F4/80) from representative lung adenoma sections; quantitation was restricted to the areas within tumor boundaries. n = 25 individual tumors from 5 KrasLA2 or 5 miR-301a−/−;KrasLA2 mice and each individual tumor was staining from 3 serial sections and quantified and averaged as three independent experiments . Values represented the mean ± s.d. of three independent experiments. **P < 0.01 or *P < 0.05 indicates a significant difference between the indicated two groups (two-tailed, unpaired Student’s t-test)