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Fig. 1 | Molecular Cancer

Fig. 1

From: Circular RNA ACVR2A suppresses bladder cancer cells proliferation and metastasis through miR-626/EYA4 axis

Fig. 1

The validation and characteristics of circACVR2A in BC cells. a Relative expression of circACVR2A in our established poorly and highly invasive T24 and UM-UC-3 cell sublines. b Relative expression of circACVR2A in immortalized uroepithelium cell line SV-HUC-1, and BC cell lines RT4, J82, 5637, UM-UC-3, T24, HT-1376, TCCSUP. c qRT-PCR analysis of circACVR2A in the reverse transcription products using random primers or oligo dT primers. d The existence of circACVR2A was detected in T24 and UM-UC-3 cell lines by qRT-PCR with convergent or divergent primers and validated by Gel electrophoresis. e The expression of circACVR2A was validated by Sanger sequencing. Red arrow represents the back-splicing site of circACVR2A. CircAVCR2A derived from back-splicing of exons 3, 4 and 5 of ACVR2A gene. f qRT-PCR analysis of circACVR2A and ACVR2A mRNA after treatment with Actinomycin D at the indicated time points in T24 and UM-UC-3 cells. g qRT-PCR analysis of circACVR2A and ACVR2A mRNA after treatment with or without RNase R in T24 and UM-UC-3 cells. h qRT-PCR analysis of circACVR2A using nuclear and cytoplasmic fractions of T24 and UM-UC-3 cells. i FISH confirmed that circACVR2A was predominantly located in cytoplasm. Nuclei were stained with DAPI. U6, 18S and circACVR2A were labeled with Cy3

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