Fig. 2

Knockdown of GBE1 promotes CD8⁺ T cell infiltration mediated by increased CCL5 and CXCL10. a Kaplan-Meier analysis of OS (calculated as years to death or years to last follow-up) using the TCGA cohort median expression value cutoffs for CD8. P-value was determined by a log-rank test. b The correlation analysis of TCGA RNA-seq data for CD8 with CCL5 or CXCL10 expression across primary LUAD samples. Pearson correlation coefficients (r) are indicated. Expression values represent log₂-transformed normalized RNA-seq readings generated with the Illumina platform. (C) Transwell assay analysis of CD8⁺ T cell migration ability in the tumor supernatants of shGBE1 A549 cells with treatment of CCL5 or CXCL10 antibodies, or shNC cells with the recombinant protein of CCL5 or CXCL10. d Quantification numbers of CD8⁺ T cells passed through the Matrigel matrix by the indicated conditions. e IHC analyses of serial sections derived from patients with LUAD (n = 30) were stained for GBE1, CD8, CCL5, CXCL10, and divided into two parts according to GBE1 high (left panel) and low expression (right panel). f IHC score of CD8, CCL5, and CXCL10 in tumor tissues from LUAD patients analyzed by IHC