Fig. 4
From: Lung adenocarcinoma-intrinsic GBE1 signaling inhibits anti-tumor immunity
Involvement of STING signaling in CCL5 and CXCL10 expression after GBE1 knockdown. a The correlation analysis of TCGA data for IFN response signature with TMEM173 expression across primary LUAD samples. b The box plots of TMEM173 expression in normal lung and tumor tissues from TCGA dataset. c Kaplan-Meier analysis of OS using the TCGA cohort median expression value cutoffs for TMEM173. The RNA-seq (d), mRNA (e) and protein (f) expression of TMEM173 in shGBE1 A549 cells compared to control. Real-time PCR (G) and western blotting (h) analysis of TMEM173 knockdown by siRNA. i The real-time PCR analysis of IFNα/β pathway expression with or without TMEM173 knockdown. Real-time PCR (j) and ELISA (k) analysis of CCL5 and CXCL10 expression with or without TMEM173 knockdown. Real-time PCR (l) and ELISA (m) analysis of CCL5 and CXCL10 in shGBE1 A549 cells compared to shNC cells with or without TMEM173 knockdown