Table 1 The sequences of primers used for quantitative real-time PCR
From: Lung adenocarcinoma-intrinsic GBE1 signaling inhibits anti-tumor immunity
Gene | Sense Primer (5′ → 3′) | Antisense Primer (5′ → 3′) |
|---|---|---|
β-actin | GCACTCTTCCAGCCTTCCTTCC | TCACCTTCACCGTTCCAGTTTTT |
GBE1 | GGAGATCGACCCGTACTTGAA | ACATCTGTGGACGCCAAATGA |
TMEM173 | AGCATTACAACAACCTGCTACG | GTTGGGGTCAGCCATACTCAG |
CCL5 | CAGTCGTCTTTGTCACCCGA | TGTAACTGCTGCTGTGTGGT |
CXCL10 | AACTGTACGCTGTACCTGCAT | GCATCGATTTTGCTCCCCTC |
PD-L1 | GGACAAGCAGTGACCATCAAG | CCCAGAATTACCAAGTGAGTCCT |
HLA-A | TGGAGAGGAGCAGAGATACACC | AGAACCAGGCCAGCAATGATG |
HLA-B | TCATCTCAGTGGGCTACGTG | GTGTGTTCCGGTCCCAATAC |
HLA-C | GGTGGTGCCTTCTGGACAAG | CTCTTCCTCCTACACATCATAGCG |
IFI27 | TGCTCTCACCTCATCAGCAGT | CACAACTCCTCCAATCACAACT |
IFI6 | CAGAAGGCGGTATCGCTTTTC | CCTGCATCCTTACCCGCATT |
IFNB1 | ATGACCAACAAGTGTCTCCTCC | GGAATCCAAGCAAGTTGTAGCTC |
IRF7 | CCCAGCAGGTAGCATTCCC | GCAGCAGTTCCTCCGTGTAG |
MX1 | AGCGGGATCGTGACCAGAT | TGACCTTGCCTCTCCACTTATC |
OASL | CTGATGCAGGAACTGTATAGCAC | CACAGCGTCTAGCACCTCTT |
STAT1 | CGGCTGAATTTCGGCACCT | CAGTAACGATGAGAGGACCCT |