Fig. 2

F-circSR promotes cell migration in NSCLC cells. a Schematic representation of pCRE5-F-circSR-expressing plasmid. b Agarose gel electrophoresis and Sanger sequencing of RT-PCR products from HEK293T cells transfected with F-circSR-expressing plasmid or empty vector. c Agarose gel electrophoresis of RT-PCR products from A549 and H1299 cells stably expressing F-circSR. d Representative images of Transwell migration assays and quantification in A549 and H1299 cells with or without F-circSR overexpression. e~f Representative images of wound healing assays in H1299 and A549 cells with or without F-circSR overexpression. g~h F-circSR knockdown attenuates the migratory ability in F-circSR1 (g) or F-circSR2 (h) overexpressing H1299 cells. SR1: F-circSR1; SR2: F-circSR2; E: exon