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Fig. 3 | Molecular Cancer

Fig. 3

From: Circular RNA circTRIM33–12 acts as the sponge of MicroRNA-191 to suppress hepatocellular carcinoma progression

Fig. 3

circTRIM33–12 binds miR-191 in HCC cells. a RIP experiments were performed using an antibody against AGO2 on extracts from Huh 7 cells. b A schematic drawing showing the putative binding sites of miR-23a-3p, miR-191, and miR-224-5p with respect to circTRIM33–12. c The luciferase activity of luc-circTRIM33–12 or mutant luc-circTRIM33–12 in Huh 7 cells after cotransfection with miR-23a-3p, miR-191, or miR-224-5p. d circRIP was performed in Huh 7 cells using biotin-labeled miR-191 mimics and a negative control (NC). The enrichment of circTRIM33–12 was detected by qRT-PCR and was normalized to that of the NC. e The expression of circTRIM33–12 in Huh 7 cells after transfection with miR-191 or miR-191 siRNA. f The expression of circ miR-191 in SMMC-7721 and Huh 7 cells after transfection with circTRIM33–12 or circTRIM33–12 shRNA. The data are represented as the mean ± SD, n = 3. *P < 0.05; **P < 0.01; NS, no significant

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