Fig. 6
circTRIM33–12 and TET1 regulate the expression of several same genes in HCC cells. a The protein levels of WWC3, TP53INP1, ULBP1 and JHDM1D were detected in SMMC-7721 cells after transfection with circTRIM33–12, TET1, or the control using western blotting. b The protein levels of WWC3, TP53INP1, ULBP1 and JHDM1D were detected in Huh 7 cells after transfection with circTRIM33–12 shRNA, TET1 shRNA, or the control using western blotting. c The luciferase activity of luc-WWC3/TP53INP1/ULBP1/JHDM1D or mutant luc-WWC3/TP53INP1/ULBP1/JHDM1D in Huh 7 cells after cotransfection with miR-191. d Representative HCC cases in the tissue microarray were analyzed by immunohistochemical staining for NKG2D. e A positive correlation between circTRIM33–12 and the number of NKG2D-positive cells was observed in HCC tissues (R2 = 0.1193; P < 0.001). The data are represented as the mean ± SD, n = 3. NS, no significant