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Fig. 2 | Molecular Cancer

Fig. 2

From: The lncRNA UCA1 promotes proliferation, migration, immune escape and inhibits apoptosis in gastric cancer by sponging anti-tumor miRNAs

Fig. 2

UCA1 functions as an onco-lncRNA promotes GC cells proliferation, migration, and inhibits apoptosis. a UCA1 overexpression GC cells were successfully established. b MTT assay was used to determine the cell viability of UCA1 overexpression and control GC cells. c Schematic diagram indicates the UCA1 knock-out vector design. Two guide RNAs targeting the promoter region of UCA1 were co-expressed by one plasmid. d UCA1 level was successfully reduced by co-transfecting UCA1-KD vector and Cas9 expression vector in two GC cells. e MTT assay to determine the cell viability of UCA1-KD GC cells. f Apoptosis assay. UCA1-KD or control GC cells were incubated with FITC labeled Annexin V antibody and then stained by PI. The percentage of apoptosis cells were determined by flow cytometry. g and h cells were deprived of serum overnight, treated with mitomycin-C and introduced into the upper chamber of the Transwell. Cells that migrated to the lower chambers were fixed with 4% paraformaldehyde and then stained with crystal violet. Crystal violet-stained cells were counted in 5 randomly different fields with an inverted microscope. Results were analyzed by student’s t-test and p < 0.05 was considered statistically significant. *p < 0.05, **p < 0.01

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