Fig. 3

UCA1 sponges miR-26a/b and promote the expression of miR-26a/b target genes. a predicted interaction between miR-26a/b and UCA1. b dual luciferase assay. HEK293T cells transfected with UCA1 3’UTR reporter vector and miR-26a/b mimic or inhibitor. Relative luciferase activity was detected 48 h post interaction. c and d miR-26a and -b levels were repressed in UCA1 overexpression GC cells, and two miR-26a and -b target genes are up-regulated. e the level of miR-26a and -b in 40 paired GC and adjacent non-tumor control samples was determined by qRT-PCR. The Correlation between UCA1 and miR-26a or -b was analyze by correlation analysis