Fig. 3
From: Pan-cancer analysis identifies telomerase-associated signatures and cancer subtypes
Knockdown of TPX2 or EXO1 decreases telomerase activities and cell viability. a Heatmaps of the degree of connectivity of genes in the cell cycle/mitotic nuclear division module, as assessed across cancers from eight different TCGA cancer types. Only hub genes exhibiting significant hub properties in at least five of the eight cancer types are shown, which we defined as consensus hub genes. TPX2, BUB1 and EXO1 are bold. b-c RT-qPCR experiments were carried out to determine the mRNA levels of endogenous TPX2 and EXO1 in HEK293T cells (b) and HepG2 cells (c) 48 h after transfection of the corresponding siRNAs. A scramble siRNA oligo was used as a control, and the results were normalized to GAPDH. d-e Relative telomerase activities were examined by Q-TRAP in TPX2- or EXO1-knockdown HEK293T cells (d) and HepG2 cells (e). Error bars indicate standard deviation, n = 3. ***P < 0.001. f-g) Representative images showing the states of HTC75 cells (F) and HepG2 cells (g) after treatment with siControl, siEXO1 or siTPX2 at 48 h and 72 h