Fig. 2
From: A novel AR translational regulator lncRNA LBCS inhibits castration resistance of prostate cancer
LBCS restores the castration sensitivity of CRPC cells. a-b Efficiency of stable overexpression of LBCS in LNCaP-AI and LNCaP-Bic, or knockdown of LBCS by two shRNAs in LNCaP cells was verified by real time qPCR. c-e The effect of LBCS overexpression on viability in LNCaP-AI and LNCaP-Bic cells, or knockdown on LNCaP cells in androgen deprived cultural medium. Cs-FBS indicates charcoal stripped fetal bovine serum. f-h The effect of LBCS overexpression on colony formation in LNCaP-AI and LNCaP-Bic cells, or knockdown on LNCaP cells in androgen deprived cultural medium. Scale bar: 200 μm. i-k LBCS overexpressed LNCaP-AI and LNCaP-Bic cells, LBCS depleted LNCaP cells were treated with different concentration of bicalutamide for 120 h and cell viability was analyzed by MTT assay. l Calculation of IC50 of bicalutamide in LNCaP-AI, LNCaP-Bic and LNCaP cells, four parameter logistic curve (best-fit solution, nonlinear regression dynamic fitting) and normality tests are used (Graph Pad Prism 6). m-o LBCS overexpressed LNCaP-AI and LNCaP-Bic cells, LBCS depleted LNCaP cells were treated with different concentration of R1881 for 120 h and cell viability was analyzed by MTT assay. The results are presented as the means ± SD of values obtained in three independent experiments. (See also Additional file 12: Figure S2) *p < 0.05, **p < 0.01