Fig. 5

HULLK positively regulates prostate cancer cell growth. a Gene structure of LCK and location of each shRNA targeting LCK. b Percent knockdown of HULLK or LCK message, n = 3. MISSION shRNAs targeting human LCK (NM_005356) and the pLKO vector control were purchased from ThermoFisher. Each shRNA was validated for efficiency of LCK or HULLK knockdown in Jurkat and PCa cells by qPCR. c CyQuant Assay measured DNA content as a surrogate for cell number 7 days after shRNA transduction. Growth was compared to untreated empty vector control and the values were averaged across biological replicates. Error bars represent standard error of the mean. The relative effect of four independent shRNAs on cell growth in LNCaP, C42, and Rv1 cells in the absence or presence of 0.05 nM R1881, n = 3. d The relative effect of HULLK overexpression on cell growth in LNCaP, C42, and Rv1 cells in the absence or presence of 0.05 nM R1881, n = 3. Statistical analysis was performed using ANOVA and Tukey test