Fig. 3

circSLC8A1 acts as a sponge for miR-130b and miR-494 in bladder cancer cells. a and b circSLC8A1 in the 5637, T24 and SV-HUC-1 lysates was pulled down and enriched with circSLC8A1 specific probe and then detected by qRT-PCR. Relative level of circSLC8A1 was normalized to the input. GAPDH was used as a negative control. c, d and e The relative levels of 7 miRNA candidates in the 5637, T24 and SV-HUC-1 lysates were detected by qRT-PCR. Multiple miRNAs can be pulled down by circSLC8A1, and both miR-130b and miR-494 were pulled down by circSLC8A1 probe in 5637 and T24 lines. f and g The biotinylated miR-130b or miR-494 was transfected into 5637 and T24 cells. After streptavidin capture, circSLC8A1 levels were quantified by qRT-PCR. h and i RNA FISH for circSLC8A1 and miR-130b/miR-494 was detected in T24. Nuclei was stained blue (DAPI), circSLC8A1 was stained red, and miR-130b/miR-494 were stained green. Data are mean ± SD, n = 3