Fig. 4

miR-130b and miR-494 promote bladder cancer progression through targeting PTEN. a and b qRT-PCR revealed that miR-130b and miR-494 were up-regulated in bladder cancer tissues (n = 30) compared with normal adjacent bladder tissues. c and d A negative correlation between the expression of circSLC8A1 and miR-130b/miR-494 was showed using Pearson correlation analysis. e and f CCK-8 assay showed that miR-130b and miR-494 promoted the proliferation of 5637 and T24 cells. g and h Transwell migration and matrigel invasion assays indicating the increased migration and invasion capabilities in 5637 and T24 cells transfected with miR-130b or miR-494 mimics. i and j The transfection of anti-miR-130b or anti-miR-494 inhibited the migration and invasion of 5637 and T24 cells. k Predicted miR-130b/miR-494 binding sites in the 3′-UTR of PTEN mRNA by bioinformatics analysis. l Luciferase reporter assay was used to confirm the interaction between miR-130b/miR-494 and PTEN mRNA. m miR-130b and miR-494 decreased the protein expression level of PTEN, individually. Data are mean ± SD, n = 3