Fig. 2
Lnc34a promoted the proliferative and motile ability of hepatoma cells in vitro and BM in vivo. a Effect of Lnc34a on cell proliferation was measured by CCK8 (n = 3). The wound-healing assay (b Magnification × 40) and transwell assay (Magnification × 100) without (c) or with Matrigel (d) were performed to analyze the effect of Lnc34a on the migration and invasion of SMMC-7721 and HCC-LM3 cells. e Bioluminescent imaging (BLI) and micro-CT of bone metastases by HCC-LM3 cells. The red arrowheads denote areas of overt osteolysis. BLI quantitation of metastasis by HCC-LM3 cells 7 weeks following an intracardiac injection (n = 11). f The representative graphs of SPECT bone scanning at seventh week of mice with metastases in mandible and vertebra. The site of bone metastasis was indicated by increased uptake of radiotracer (99mTc-MDP), with characteristic radioactivity accumulation in the joints and metabolism of urinary system including bladder. g Representative H&E-stained sections (T, tumor; C, cortical bone; M, normal bone marrow). h Osteoclast TRAP staining. NC, negative control. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001