Fig. 3

Lnc34a suppresses miR-34a expression by miR-34a promoter methylating and histone deacetylating (n = 3). a Ectopic Lnc34a suppresses miR-34a expression in SMMC-7721 cells and knock-down of Lnc34a increases miR-34a levels in HCC-LM3 cells. b The location of Lnc34a and miR-34a in SMMC-7721 and HCC-LM3 cells, as determined by RNA-FISH (Magnification: × 200). c Bisulfate-sequencing PCR results showing that the knock-down of Lnc34a decreased methylation on the miR-34a promoter in HCC-LM3 cells, while the over-expression of Lnc34a induced the hypermethylation of the miR-34a promoter. d The association between the acetylated histones H3 or H4 and the miR-34a promoter was decreased in SMMC-7721 cells with the Lnc34a ectopic expression using ChIP-qPCR with antibodies against the acetylated histones H3 and H4, whereas the opposite result was found in HCC-LM3 cells with the knockdown expression of Lnc34a. **P < 0.01; ***P < 0.001; ****P < 0.0001