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Fig. 7 | Molecular Cancer

Fig. 7

From: The molecular mechanism of LncRNA34a-mediated regulation of bone metastasis in hepatocellular carcinoma

Fig. 7

miRNA-34a inhibits BM in HCC both in vitro and in vivo. a Typical images of miR-34a by ISH and Smad4 by IHC. Magnification: × 200. b BLI and micro-CT of BM by HCC-LM3 cells transfected with miR-34a, Smad4, miR-34a plus Smad4 or NC. Arrowheads denote areas of overt osteolysis. BLI quantitation of metastasis 7 weeks after intracardiac injection (n = 8). c The representative graphs of SPECT bone scanning at seventh week of mice with metastases in tibia, mandible and lumbar vertebrae, indicated by increased accumulation of 99mTc-MDP. d and e Representative H&E images, osteoclast TRAP staining, and IHC analyses of BM genes (Magnification: × 200). Arrows point to TRAP+ osteoclasts along the tumor-bone interface. NC, negative control. *P < 0.05; **P < 0.01

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