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Fig. 1 | Molecular Cancer

Fig. 1

From: Circular RNA circRHOT1 promotes hepatocellular carcinoma progression by initiation of NR2F6 expression

Fig. 1

circRHOT1 is overexpressed in HCC tissues. a Heatmap of upregulated circRNAs in HCC tissues according to Han’s and Fu’s cohorts. circRHOT was both significantly upregulated and conserved in the two cohorts. b The relative expression levels of circRHOT in tumor tissues and adjacent peri-tumor tissues from 100 pairs of HCC patient samples. c circRHOT expression was analyzed by northern blotting using 5 paired HCC samples. P, peri-tumor; T, tumor. circRHOT1 was detected using backsplice junction probe. RHOT1 mRNA was detected using RHOT1 specific probe. 18S was a loading control and RNA samples for 18S detection were not treated with RNase R. d, e The expression of circRHOT was determined by in situ hybridization (ISH) (d) and RNA fluorescence in situ hybridization (RNA FISH) (e) using HCC samples #1 and #4. The scale bars for ISH were 50 μm. The scale bars for FISH were 10 μm. f The expression of circRHOT was examined in different clinical stages of HCC samples by qRT-PCR. g The relative expression levels of circRHOT in the peritumor, early HCC (eHCC) and advanced HCC (aHCC) were determined by qRT-PCR. h, i The 100 HCC samples were divided into high and low groups based on circRHOT expression. Next, Kaplan–Meier survival analyses were conducted to determine the overall survival rate (h) and recurrence-free survival rate (i). *p < 0.05, **p < 0.01 and ***p < 0.001. All the data are representative of at least three independent experiments and presented as the means ± SD

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