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Fig. 3 | Molecular Cancer

Fig. 3

From: A novel tumor suppressor protein encoded by circular AKT3 RNA inhibits glioblastoma tumorigenicity by competing with active phosphoinositide-dependent Kinase-1

Fig. 3

Tumor suppressive functions of AKT3-174aa in glioma cell lines. a U251, U373 glioma cells were transfected with circ-AKT3, linearized AKT3-174aa and control vectors; SW1783, Hs683 glioma cells were transfected with circ-AKT3 shRNA or control shRNA. AKT1, AKT2, AKT3 and AKT3-174aa level were determined by immunoblot. b Upper, cell proliferation, EdU incorporation of circ-AKT3, linearized AKT3-174aa transfected U251 and U373 cells and their control cells were decided. Lower, cell proliferation, EdU incorporation of circ-AKT3 shRNA transfected SW1783 and Hs683 cells and their control cells were decided. Error bars represent three independent experiments, *, p < 0.05, **, p < 0.01, ***, p < 0.001. c Left, plate colony formation and anchorage independent growth of circ-AKT3, linearized AKT3-174aa transfected U251 and U373 cells and their control cells were decided. Right, plate colony formation and anchorage independent growth of circ-AKT3 shRNA transfected SW1783 and Hs683 cells and their control cells were decided. Error bars represent three independent experiments, *, p < 0.05.d Upper, radiation resistance (cell cytometry or H2AX foci counting followed by 12Gy radiation) of circ-AKT3, linearized AKT3-174aa transfected U251 and U373 cells and their control cells were decided. Lower, radiation resistance (cell cytometry followed by 12Gy radiation) of circ-AKT3 shRNA transfected SW1783 and Hs683 cells and their control cells were decided. Error bars represent three independent experiments, *, p < 0.05

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