Fig. 4

AKT3-174aa, but not circ-AKT3, exerts the biological function. a SW1783 and Hs683 cells were transfected with circ-AKT3 shRNA or circ-AKT3 shRNA plus linearized AKT3-174aa overexpression plasmid. AKT3-174aa expression was decided by immunoblot. b Cell proliferation and cell cycle analysis of SW1783 and Hs683 expressing circ-AKT3 shRNA, circ-AKT3 shRNA plus linearized AKT3-174aa and their control cells. Error bars represent three independent experiments, *, p < 0.05, **, p < 0.01, ***, p < 0.001. c Plate colony formation and anchorage independent growth of SW1783 and Hs683 expressing circ-AKT3 shRNA, circ-AKT3 shRNA plus linearized AKT3-174aa and their control cells. Error bars represent three independent experiments, *, p < 0.05. d Radiation resistance (cell cytometry or H2AX foci counting followed by 12Gy radiation) of SW1783 and Hs683 expressing circ-AKT3 shRNA, circ-AKT3 shRNA plus linearized AKT3-174aa and their control cells. Error bars represent three independent experiments, *, p < 0.05. e U251 and U373 cells were transfected with linearized AKT3-174aa plasmid or ATG mutated circ-AKT3 plasmid, and AKT3-174aa was determined by immunoblot. f Cell proliferation and cell cycle analysis of U251 and U373 cells transfected with linearized AKT3-174aa plasmid or ATG mutated circ-AKT3 plasmid. Error bars represent three independent experiments, *, p < 0.05, **, p < 0.01. g Plate colony formation and anchorage independent growth of U251 and U373 cells transfected with linearized AKT3-174aa plasmid or ATG mutated circ-AKT3 plasmid. Error bars represent three independent experiments, *, p < 0.05. h Radiation resistance (cell cytometry or H2AX foci counting followed by 12Gy radiation) of U251 and U373 cells transfected with linearized AKT3-174aa plasmid or ATG mutated circ-AKT3 plasmid. Error bars represent three independent experiments, *, p < 0.05, **, p < 0.01