Table 1 Primers and oligos used
RT-PCR primers | |||
|  | Forward primer(5‘to 3’) | Reverse primer(5‘to 3’) | Amplified product(bps) |
 QPCR Circ-AKT3(Divergent primers) | AAGTGGCACACACTCTAACTG | GTTTTCATTAACTGGCATTCTCG | 150 |
 QPCR Linear-AKT3(Convergent primers) | GGAGTCATCATGAGCGATGT | TAACTGGCATTTTGCCACTG | 192 |
 QPCR-AKT1 | CATGAGCGACGTGGCTATTG | GCCTCACGTTGGTCCACATC | 150 |
 QPCR-AKT2 | AAGAAGGCTGGCTCCACAAG | GCATTCTGCTACGGAGAAGT | 158 |
 QPCR-beta-actin | ACAGAGCCTCGCCTTTGCCGAT | CTTGCACATGCCGGAGCCGTT | 109 |
Plasmid construction primers | |||
 OV-circ-AKT3 | TTCGAATTCAGTGCTGAGATTACAGGCGTGAG | TTCGAATTCAGTGCTGAGATTACAGGCGTGAG | 686 |
 OV-AKT3-174aa-flag | TTCGAATTCATGAAAACAGAACGACCAAAGC | AATGGATCCTTACTTGTCATCGTCATCCTT | 609 |
 Rluc-PCR | AGGCTAGCGCCACCATGGCTTCCAAGGTGT | TTATTACTGCTCGTTCTTCAGCAC | 953 |
 luc-PCR | GCCACCATGGCCGATGCTAA | CGCTCGAGTTACACGGCGATCTTGCCGCCTT | 1667 |
 Circ-AKT3-IRES-WT | ATGGTACCAATGGACAGAAGCTATCCAGGCTGTA | CTGGAATTCCCTTCTCTCGAACCAAAATAACT | 233 |
 Circ-AKT3-IRES-Del-1 | ATGGTACCAATGGACAGAAGCTATCCAGGCTGTA | CTGGAATTCATCTCTTCCTCTCCTATATTATCAA | 126 |
 Circ-AKT3-IRES-Del-2 | ATGGTACCTGGATGCCTCTACAACCCATCA | CTGGAATTCCCTTCTCTCGAACCAAAATAAC | 125 |
Probes | |||
 Circ-AKT3-FISH-probe | Cy3-TTTCATTAACTGGCATTCTCGCCCCCATTAAC |  |  |
 Northern-Circ-AKT3-probe | TTTCATTAACTGGCATTCTCGCCCCCATTAAC-DIG |  |  |
 Northern-U6-probe | TCTTCTCTGTATCGTTCCAATTTTAGTATATGTGC-DIG |  |  |
siRNA sequences | |||
 Name | sense(5'-3') | antisense(5'-3') |  |
 Circ-AKT3-siRNA-1 | GGGGCGAGAAUGCCAGUUAAUtt | AUUAACUGGCAUUCUCGCCCCtt |  |
 Circ-AKT3-siRNA-2 | GGCGAGAAUGCCAGUUAAUGAtt | UCAUUAACUGGCAUUCUCGCCtt |  |
 Circ-AKT3-siRNA-NC | GCGCCCUGAUUGCCUGAAAUAtt | UAUUUCAGGCAAUCAGGGCGCtt |  |