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Fig. 2 | Molecular Cancer

Fig. 2

From: Circular RNA circ-ZKSCAN1 inhibits bladder cancer progression through miR-1178-3p/p21 axis and acts as a prognostic factor of recurrence

Fig. 2

Characterization of Circ-ZKSCAN1 in BCa cells. a The expression of circ-ZKSCAN1 in wild-type, highly and lowly invasive baldder cancer cell sublines was detected by qPCR. b qPCR was used to examine the expression of circ-ZKSCAN1 in SV-HUC-1, 5637, EJ, UM-UC-3 and T24 cells. c Nuclear mass separation assay showed that circ-ZKSCAN1 was predominantly localized in cytoplasm. β-actin was mostly localized in cytoplasm and U6 was mainly localized in nucleus, which used as a negative control. d Fluorescence in situ hybridization (FISH) also showed that circ-ZKSCAN1 was predominantly localized in cytoplasm. The abbreviations of circ-ZKSCAN1 is cZKSCAN1

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