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Fig. 5 | Molecular Cancer

Fig. 5

From: Circular RNA circ-ZKSCAN1 inhibits bladder cancer progression through miR-1178-3p/p21 axis and acts as a prognostic factor of recurrence

Fig. 5

Circ-ZKSCAN1 may function as a sponge of miR-1178-3p. a Schematic model shows the putative binding sites of 12 miRNA candidates correlated with circ-ZKSCAN1 through miRanda and TargetScan. b The relative expressions of 12 miRNAs in UM-UC-3 was detected by qPCR. miR-1178-3p, miR-29b-3p and miR-765 was significantly enriched in the probe of circ-ZKSCAN1. c Luciferase reporter assay in HEK293T cells co-transfected with miR-1178-3p mimics, empty vector or the vector contained sequence of circ-ZKSCAN1. Data were calculated by hRluc to hLuc+. d Luciferase reporter assay in HEK293T cells co-transfected with miR-1178-3p mimics and psiCHECK-2-mutant type circ-ZKSCAN1 (circ-ZKSCAN1-mut) plasmids. Data were calculated by hRluc to hLuc+. e Schematic of circ-ZKSCAN1 wild-type (wt) and mutant (mut) luciferase reporter vectors. f FISH assay showed that circ-ZKSCAN1 was co-localized with miR-1178-3p in cytoplasm

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Molecular Cancer

ISSN: 1476-4598