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Fig. 4 | Molecular Cancer

Fig. 4

From: LncRNA SATB2-AS1 inhibits tumor metastasis and affects the tumor immune cell microenvironment in colorectal cancer by regulating SATB2

Fig. 4

SATB2-AS1 exerts its biological roles by regulating SATB2. a, Transwell assays were used to determine the invasion and migration abilities of CRC cells with the indicated treatment. b, Upper panel, representative CT scans, images of the gross lesion in lung tissues and HE staining of metastatic nodules in the lungs from the indicated group. Lower panel, summarized data on metastatic rates and survival analysis was performed in mice bearing CRC transfected with corresponding cells. c, Upper panel, representative images of SATB2-AS1 expression in CRC tissues detected by ISH and SATB2, E-cadherin, and Vimentin expression in CRC tissues detected by IHC in our CRC cohort 2. Lower panel, scatter plot showing the expression relationship among SATB2-AS1, SATB2, E-cadherin and Vimentin in our CRC cohort 2. The upper right squares show the Pearson correlations among them. d, SATB2, E-cadherin, Vimentin and MMP2 were detected by western blot in CRC cells with the indicated treatment. e, CXCL9 and CXCL10 were detected by qRT-PCR (left) and western blot (right) in HCT-116 cells with the indicated treatment. Scale bar = 20 μm. *, P < 0.05; **, P < 0.01 and ***, P < 0.001

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