Fig. 6

Simultaneous inhibition of PVT1 and YAP1 by their ASOs had better anti-tumor activity on EAC cells in vitro and in vivo. a. Expression of PVT1(left) and YAP1 (right) were detected in JHESO and OE19 cells treated with PVT1 ASOs, YAP1 ASOs or their combination by real-time q-PCR. b. Colony formation of JHESO (left) and OE19 (right) cells were significantly suppressed by PVT1 and YAP1 ASOs alone or in combination. c. Average tumor volume were demonstrated in mice that treated with PVT1 ASO, YAP1 ASO and their combination for 4 weeks. d. Tumors weights at the termination of the experiment were shown. e & f. Representative tumors (e) and mice weight (f) after 4 weeks were calculated as described as Materials&Methods. Error bars, mean ± SEM. E. *, P < 0.05; **, P < 0.01. g. Proposed model by which mutual regulation of PVT1 and YAP1