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Fig. 2 | Molecular Cancer

Fig. 2

From: METTL3-mediated N6-methyladenosine modification is critical for epithelial-mesenchymal transition and metastasis of gastric cancer

Fig. 2

Gain of mesenchymal features and simultaneous loss of epithelial characteristics correlate with METTL3 expression in GC. a Representative IHC staining of EMT markers in pathologic serial sections of GC tissues with low or high METTL3 expression. Scale bars: 200 μm. b Western blot analysis of METTL3 and EMT markers in different cell lines. c The shRNA-mediated METTL3 repression was confirmed by qRT-PCR after lentivirus infection in BGC823 and AGS cells. d Overexpression of METTL3 was confirmed by qRT-PCR after lentivirus infection in MKN-28 cells. e METTL3 knockdown or overexpression influenced the expression levels of EMT markers in the western blot assay. f-g The global m6A modification levels in METTL3 knockdown and overexpression GC cells determined by LC-MS/MS analysis. **p < 0.01, ***p < 0.001

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