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Fig. 7 | Molecular Cancer

Fig. 7

From: METTL3-mediated N6-methyladenosine modification is critical for epithelial-mesenchymal transition and metastasis of gastric cancer

Fig. 7

Transcription repression of E-cadherin by ZMYM1-CtBP/LSD1/CoREST complex. a-b BGC823 and MKN-28 cells were transfected with different amounts of Gal4-ZMYM1 (Flag-tagged) expression plasmids, together with the Gal4-luciferase reporter. Relative luciferase activity was measured after 48 h. c MKN-28 cells were transfected with Flag-ZMYM1 plasmids together with the luciferase reporter. Relative luciferase activity was measured after 48 h. d-e Whole-cell lysates from BGC823 and MKN-28 cells were immunoprecipitated with antibodies against ZMYM1 or IgG, followed by immunoblotting with the antibodies against CtBP1/2, LSD1 and CoREST. f Whole-cell lysates from GC cells were immunoprecipitated with antibodies against CtBP1/2, LSD1 and CoREST followed by immunoblotting with the antibodies against ZMYM1. g ZMYM1 associated complex bound specifically to the E-cadherin proximal promoter in MKN-28 cells. h Co-occupancy of the E-cadherin promoter by ZMYM1 and the components of CtBP/LSD1/CoREST complex. i The protein level of E-cadherin was determined by western blot analysis after transfection of si-CtBP1/si-LSD1/si-CoREST, together with ZMYM1 expression construct. *p < 0.05, **p < 0.01

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