Fig. 8

METTL3-mediated epigenetic activation of ZMYM1 is responsible for EMT and metastasis of GC. a The protein levels of EMT markers were measured by western blot analysis in BGC823 cells transfected with lentiviruses carrying sh-METTL3 and/or ZMYM1. b The protein levels of EMT markers were measured by western blot analysis in MKN-28 cells transfected with lentiviruses carrying METTL3 and/or sh-ZMYM1. c-f The invasive abilities were explored by transwell assay in GC cells transfected with lentiviruses carrying sh-METTL3 and/or ZMYM1, or transfected with lentiviruses carrying METTL3 and/or sh-ZMYM1. g-h Lung metastatic assay was performed to determine whether ZMYM1 enhanced or inhibited the metastatic abilities of BGC823 cells or MKN-28 cells by repressing or overexpressing METTL3 expression in nude mice, respectively. i-j ZMYM1 expression was reduced with decreased lung metastasis burden and upregulated with more lung colonization caused by modified METTL3 expression according to IHC analysis. Scale bars: 100 μm. *p < 0.05, **p < 0.01, ***p < 0.001