Fig. 5
Salinomycin restrains hepatic metastasis in UM. a Photomicrograph of the wound healing scratch assay from control and salinomycin (1.25 μmol/L)-treated UM cells is shown. Scale bar: 200 μm. b and c Twenty-four hours after incubated with 1.25 μmol/L salinomycin, viable cells were counted and underwent transwell (b) or matrigel invasion chamber assays (c). Representative images (left) for UM cells and quantitative analysis (right) from three random microscopic fields are shown. Data represent mean ± SD. ***, P < 0.001, Student’s t test. Scale bar: 200 μm. d After intrasplenic injection of 5 × 105 of Mel270-Luc cells, the NOG mice were administrated vehicle (corn oil) or salinomycin (i.p., 2.5 mg/kg, QD) every day for 21 days. In vivo bioluminescence imaging of hepatic metastasis after administration with salinomycin. Left, representative images of luciferase signals on day 21 after administration with vehicle (n = 4) or salinomycin (n = 4). Right, quantification of photon flux for hepatic metastases in NOG mice was performed every week. Data represent mean ± SD. ns, not significant; *, P < 0.05; ***, P < 0.001, Student’s t test. e Photomacrograph of liver was taken from the mice received intrasplenic injection of 5 × 105 of Mel270-Luc cells and administration with vehicle or salinomycin for 21 days. Right, representative liver images are shown. Left, surface metastatic nodules in the liver from each group were counted. Data represent mean ± SD. *, P < 0.05, Student’s t test. f H&E staining in liver tissue sections. Scale bar: 500 μm. Right, representative H&E staining images in liver tissue sections are shown. Left, the number of micrometastasis in microscopic fields of each mice were counted. Data shown are the mean ± SD of number of micrometastasis in microscopic fields from 4 mice. **, P < 0.01, Student’s t test