Fig. 7

Salinomycin impedes traits of CSCs at least partially through suppressing Twist1. a-c 92.1 cells transduced with retroviral Twist1 cDNA-expressing constructs were incubated in the presence or absence of salinomycin for 24 h, and then subjected to assays of Western blot (a), Aldefluor assay (b) and melanosphere (c), respectively. Representative images for UM cells and quantitative analysis from three independent experiments are shown. Data represent mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; one-way ANOVA, post hoc comparisons, Tukey’s test. d-f 92.1 cells stably transduced with lentiviral Twist1-shRNA constructs were treated with or without 2.5 μmol/L salinomycin for 24 h, and then subjected to Western blot (d), Aldefluor assay (e), melanosphere assays (f), respectively. Representative images for UM cells and quantitative analysis from three independent experiments are shown. Data represent mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; Student’s t test. g and h UM cells transduced with retroviral constructs of Twist1-encoding or lentiviral shRNA against Twist1 were exposed to 2.5 μmol/L salinomycin for 24 h, then underwent transwell migration and matrigel invasion assay. *, P < 0.05; **, P < 0.01; ***, P < 0.001; one-way ANOVA, post hoc comparisons, Tukey’s test for g and Student's t test for h