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Fig. 1 | Molecular Cancer

Fig. 1

From: Circ-AKT3 inhibits clear cell renal cell carcinoma metastasis via altering miR-296-3p/E-cadherin signals

Fig. 1

circRNAs expression profiles of ccRCC and characteristic of circ-AKT3. a Scatter plot compared the expression fold changes of circRNAs for ccRCC tissues versus adjacent normal kidney tissues. The green lines are Fold Change Lines. The circRNAs above the top green line and below the bottom green line indicated more than 2.0 fold change of circRNAs between the two compared groups. b Clustered heatmap for deregulated circRNAs in ccRCC (filtered by |FC (fold change)| ≥ 2 and P < 0.05), with rows representing tissues and columns representing circRNAs. The numerical data represented the circRNA in Arraystar. c Schematic illustration of circ-AKT3, the genomic structure shows that the sequence of circ-AKT3 contains seven exons (808 bp) from the AKT3 gene. Outward-facing primers (F/R) were designed against circ-AKT3 to examine its expression. d and e RT-qPCR analysis of expression fold change for circ-AKT3 in unpaired and paired ccRCC tissues compared with adjacent normal kidney tissues. f Expression level of circ-AKT3 in ccRCC cell lines were screened by qRT-PCR. OSRC-2, A498 and SM12-PN6 exhibited higher circ-AKT3 expression, while others are circ-AKT3-low-expressed. g The expression levels of circ-AKT3 and linear AKT3 mRNA were determined by qRT-PCR after RNase R treatment. Data are the means ± SEM of three independent experiments. **P < 0.01; ***P < 0.001

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