Fig. 2

circ-AKT3 inhibits ccRCC cells migration and invasion in vitro. a Three siRNAs were designed to silence circ-AKT3 by targeting the spliced junction of circ-AKT3, it revealed that all the three siRNAs could downregulate the expression level of circ-AKT3 but had no effect on that of linear AKT3. Specially, si-circ-AKT3 #2 showed the highest silencing efficiency. b The plasmid over-expressing circ-AKT3 was constructed and its transfection efficiency was verified by qRT-PCR. c The cell migration capability was evaluated by the Wound healing assay in OSRC-2 cells transfected with si-Negative control (NC) and si-circ-AKT3 #2 respectively. d-e Transwell migration and invasion assays were performed to determine the role of circ-AKT3 in OSRC-2. f The cell migration capability was evaluated by the Wound healing assay in SW839 cells transfected with Vector and circ-AKT3-overexpressing plasmid respectively. g-h Transwell migration and invasion assays were performed to determine the role of circ-AKT3 in SW839. Data are the means ± SEM of three independent experiments. **P < 0.01; ***P < 0.001