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Fig. 5 | Molecular Cancer

Fig. 5

From: Circ-AKT3 inhibits clear cell renal cell carcinoma metastasis via altering miR-296-3p/E-cadherin signals

Fig. 5

miR-296-3p mediates circ-AKT3-dependent E-cadherin expression and cell migration and invasion. a-b Transwell analysis after the co-transfection of miR-296-3p mimics and circ-AKT3 in OSRC-2 cells. c Western blotting analysis showed that miR-296-3p could decrease the protein level of E-cadherin and co-transfection of circ-AKT3 could reverse this effect in OSRC-2 cells. d-f Transwell and Western blotting analysis after the co-transfection of miR-296-3p mimics and circ-AKT3 in SW839 cells. g-j The luciferase reporter assay was used to detect the luciferase activity of pmirGLO-circAKT3wildtype/mutant and pmirGLO-E-cadherin-wildtype/mutant in OSRC-2 and SW839 cells co-transfected with miR-296-3p mimics, and the matched and mutant sequences were all shown. Data are the means ± SEM of three independent experiments. **P < 0.01; ***P < 0.001

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Molecular Cancer

ISSN: 1476-4598