Fig. 1
From: Exosomal miR-21 regulates the TETs/PTENp1/PTEN pathway to promote hepatocellular carcinoma growth
Characterization of exosomes and the expression of miR-21, PTENp1 and PTEN. a High-resolution transmission electron microscopic image of SNU-449 cell exosomes (scale bar = 100 nm). b Western blot analysis showing the expression of exosomal biomarkers in HCC cells and exosomes. c The size distribution of isolated exosomes. d MiR-21 expression in noncancerous primary human hepatocytes (normal), HCC cells and exosomes was measured by real-time qPCR analysis. e After transfection of miR-21 inhibitors or addition of GW4869, miR-21 expression in SNU-449 cells and exosomes was measured by real-time qPCR analysis. f PTEN and PTENp1 expression in hepatocytes and HCC cells was measured by real-time qPCR analysis. g and h PTEN expression was measured by western blot analysis. The values expressed are the mean ± SD from three samples (*P < 0.01, vs. normal or control)