Fig. 8

Effect of exosomal miR-21 on tumor formation in nude mice. a Mice were transplanted with SNU-449 cells and exosomes, as follows. miR-21I-SNU-449 (miR-21 I-SNU): Nude mice were inoculated subcutaneously with SNU-449 cells transfected with miR-21 inhibitors. SNU-449: Nude mice were inoculated subcutaneously with SNU-449 cells. SNU-449 + Exosomes (SNU-449 + Exo): Nude mice were inoculated subcutaneously with SNU-449 cells, and then SNU-449-derived exosomes were directly administered via intratumor injection. SNU-449 + Exosomes+miR-21 inhibitors (SNU + Exo + miR-21 I): Nude mice were inoculated subcutaneously with SNU-449 cells, and then exosomes transfected with miR-21 inhibitors were directly administered via intratumor injection. SNU-449 + miR-21 inhibitors-Exosomes (SNU + miR-21 I-Exo): Nude mice were inoculated subcutaneously with SNU-449 cells, and then exosomes derived from SNU-449 cells that had been transfected with miR-21 inhibitors were directly administered via intratumor injection. b The volumes of tumors after transplantation. ^*P < 0.01, vs. miR-21 I-SNU. c miR-21, PTEN, PTENp1, TET1, TET2 and TET3 expression in tumor tissues was measured by real-time qPCR analysis. d and e PTEN, TET1, TET2 and TET3 expression in tumor tissues was measured by western blot analysis. f Akt and p-Akt expression in tumor tissues was measured by western blot analysis. Each bar represents the mean ± SD determined from three samples (^*P < 0.01, vs. SNU-449)