Table 1 Sequences of primers and siRNAs
From: Exosomal miR-21 regulates the TETs/PTENp1/PTEN pathway to promote hepatocellular carcinoma growth
Name | sense (5′-3′) | antisense (5′-3′) |
|---|---|---|
Primer set for real-time PCR | ||
GAPDH | AGGTCGGTGTGAACGGATTTG | TGTAGACCATGTAGTTGAGGTCA |
PTEN | CTTACAGTTGGGCCCTGTACCATCC | TTTGATGCTGCCGGTAAACTCCACT |
PTENp1 | GGATCATTACCTCACACCATACC | TCTAAGAAACAACTAAGCCAAAGTC |
TET1 | TCTGTTGTTGTGCCTCTGGA | GCCTTTAAAACTTTGGGCTTC |
TET2 | GAGACGCTGAGGAAATACGG | TGGTGCCATAAGAGTGGACA |
TET3 | CCCACAAGGACCAGCATAAC | CCATCTTGTACAGGGGGAGA |
MS QPCR primers | ||
unmethylated PTENp1 | TAGTAGTGAGAATATTTGGATATAGGGC | AATTTACTACACCGATTAACTCGTC |
methylated PTENp1 | GAGAATATTTGGATATAGGGTGG | AATTTACTACACCAATTAACTCATC |
ChIP-PCR primers | ATTACGAACCTAGAAGATGCTCTC | CTCACAGCGGCTCAACATTC |
GlucMS-qPCR primers | ACGAACCTAGAAGATGCTCTCCTC | AGCCCGGCCTCGCCTCAC |
miR-21 inhibitor | UAGCUUAUCAGACUGAUGUUGA | AUCGAAUAGUCUGACUACAACU |
siRNAs | ||
PTENp1 | ATCAGAGATCATATAGGAATA | |
TET1 | CCAGTCTTAATAAGGTTAT | |
TET2 | CAAGACCAATGTCAGAA | |
TET3 | GATGAAGGTCCATATTA | |
Negative control | AACAGTCGCGTTTGCGACTGG | |