Fig. 2

Knockdown of circMYO10 inhibits osteosarcoma cell proliferation and migration, and induces cell cycle arrest. a Transwell migration and invasion assays demonstrated that downregulation of circMYO10 compromises the cell migration and invasion abilities in MG63 and U2OS cells. Data represents the mean ± SD (n = 3). Scale bars = 50 μm. b CircMYO10 knockdown suppresses cell migration in MG63 and U2OS cells as evaluated by a wound healing assay. Data represents the mean ± SD (n = 3). Scale bars = 200 μm. c CircMYO10 knockdown inhibits colony formation in both MG63 and U2OS cells. Data represents the mean ± SD (n = 3). d Knockdown of circMYO10 inhibited cell proliferation as indicated by CCK-8 assays in MG63 and U2OS cells. Data represents the mean ± SD (n = 18). e Downregulation of circMYO10 arrested MG63 and U2OS cells at the G0/G1 phase. Data represents the mean ± SD (n = 3). f CircMYO10 knockdown inhibited anchorage-independent colony formation of both MG63 and U2OS cells. Scale bars = 50 μm. g The protein expression of N-cadherin, E-cadherin, Vimentin, and cyclinD1 was detected by western blot analysis in both MG63 and U2OS cells transfected with SicircMYO10. Three independent assays were performed in the above assays. a-e * P < 0.05, ** P < 0.01, *** P < 0.001 (Student’s t-test)