Fig. 8
CircMYO10 acts as a miR-370-3p sponge to promote the growth and metastasis of OS cells in vivo. a Nude mice were injected subcutaneously with 107 stable MG63 control cells or cells transfected with shcircMYO10 or cells co-transfected with circMYO10 shRNA and miR-370-3p sponge. Tumors were dissected, photographed, and weighed after 5 weeks. b Tumor volume was measured every week. Data represents the mean ± SD (n = 6). c Average tumor weight in each group at the end of the experiment (day 35). Data represents the mean ± SD (n = 6). d Western blot analysis of RUVBL1, β-catenin, C-myc, CyclinD1, Vimentin, E-cadherin, and N-cadherin of protein extracted from tumors. Four tumors were selected from each group for western blot analysis. Sh: shcircMYO10. Sh + sponge: shcircMYO10 + miR-370-3p sponge. e Immunohistochemistry analysis of RUVBL1, β-catenin, C-myc, CyclinD1, N-cadherin, Vimentin, and E-cadherin in tumors. Scale bars = 100 μm. f Representative images of Bioluminescence imaging of in vivo metastatic activity in nude mice 4 weeks after vail vein injection. g Model patterns of circMYO10/miR-370-3p/RUVBL1 axis. Three independent assays were performed in the above assays. b-c, f * P < 0.05, ** P < 0.01, *** P < 0.001 (Student’s t-test)