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Fig. 4 | Molecular Cancer

Fig. 4

From: ASPH-notch Axis guided Exosomal delivery of Prometastatic Secretome renders breast Cancer multi-organ metastasis

Fig. 4

ASPH activates Notch signaling pathway to spur metastasis in orthotopic murine model. (a) Compared to empty vector, MDA-MB-231 cells stably expressing ASPH exhibited high tumorigenicity in orthotopic model (n = 5/group). Increased primary tumor weight illustrated tumor development was bolstered by ASPH. Anti-tumor effect of MO-I-1182 was notable in tumors generated from MDA-MB-231 cells with exogenous ASPH. *p < 0.05; **p < 0.01. (b) Gross appearance of the lungs derived from representative mice in orthotopic model. Metastatic lesions were highlighted with yellow arrows. (c) Histologic characteristics of pulmonary metastases. (d) Gross appearance and histologic characteristics of (Upper) pulmonary and (Lower) axillary lymph nodes metastatic lesions of two representative mice in orthotopic model. Metastatic lesions maintained high expression of ASPH. These mice were orthotopically injected with MDA-MB-231 stably expressing ASPH and treated with DMSO. (e) Gross appearance of the invaded organs by primary breast cancer derived from representative mice in orthotopic model (n = 5/group). Metastatic lesions were highlighted with yellow arrows. (F-G) Expression profiling of key components in Notch signal pathway, including activated Notch1 (ICD), ADAM17, downstream MMPs, was downregulated by the SMI. *p < 0.05; **p < 0.01

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