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Fig. 4 | Molecular Cancer

Fig. 4

From: Circular RNA FOXP1 promotes tumor progression and Warburg effect in gallbladder cancer by regulating PKLR expression

Fig. 4

circFOXP1 interacts with PTBP1 in GBC cells. a A RNA pull-down assay was performed (left) and the RNA-related proteins were determined with SDS-polyacrylamide gel electrophoresis (SDS/PAGE) and silver staining (right). b PTBP1 was pulled down by a circFOXP1 sense RNA probe but not by an antisense RNA probe or no RNA in NOZ and SGC-996 cells. Western blot analysis was performed to detect the specific association of PTBP1 and circFOXP1 (n = 3). c RIP assays with qRT-PCR (top) or RT-PCR (low) showed that circFOXP1 was pulled down by an anti-PTBP1 antibody in NOZ and SGC-996 cells (n = 3). d The relative protein expression of PTBP1 after transfected with sh-NC, sh-circFOXP1–1 or sh-circFOXP1–2 in NOZ and SGC-996 cells and transfected with pLCDH-vector or pLCDH-circFOXP1 in GBC-SD cells were determined by western blot analysis (n = 3). All data are shown as mean ± S.E.M., n = 3 or more, **P < 0.01

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