Fig. 2

CircPLEKHM3 exerts tumor-suppressive effects in ovarian cancer cells. a The relative abundance of circPLEKHM3 in ovarian cancer cells transfected with circPLEKHM3 and mock plasmids determined by qRT-PCR. b Proliferation of A2780 and MDAH2274 cells transfected with circPLEKHM3 overexpression and mock vectors assessed with the CCK8 kit. c Migration of A2780 and MDAH2274 cells transfected with circPLEKHM3 overexpression and mock vectors measured in a transwell assay at 24 h. d Immunoblotting of E-cadherin, ZO-1, Slug and SNAIL1 in A2780 and MDAH2274 cells transfected with circPLEKHM3 overexpression and mock vectors. e qRT-PCR analysis of the relative abundance of circPLEKHM3 in OV90 and A2780 cells transfected with two siRNAs and scrambled control. f Proliferation of A2780 and MDAH2274 cells transfected with two siRNAs targeting circPLEKHM3 and scrambled control assessed with the CCK8 kit. (G) Migration of A2780 and OV90 cells transfected with two siRNAs targeting circPLEKHM3 and scrambled control measured in a transwell assay. h Immunoblotting for E-cadherin, ZO-1, Slug and SNAIL1 in A2780 and OV90 cells transfected with siRNA1. i Representative images in immunodeficient mice. A2780 scramble and sh-circPLEKHM3 cells were injected subcutaneously in the left and right flank of mice, respectively. j The growth curves for tumors and tumor weights of immunodeficient mice injected with A2780 scramble cells and A2780 sh-circPLEKHM3 cells. Tumor volumes were normalized by the first measurement at day 7 after injection. Absolute tumor weights were measured after mice were sacrificed 3 weeks after injection. Six mice per group were used in the nude mouse assay