Fig. 2
From: circLMTK2 acts as a sponge of miR-150-5p and promotes proliferation and metastasis in gastric cancer
Characteristics of the circLMTK2 in GC cells. (a) The genomic loci of the LMTK2 gene and circLMTK2. The expression of circLMTK2 was detected by qRT-PCR and was validated by Sanger sequencing. The arrows represent divergent primers that bind to the genomic region of circLMTK2. (b) qRT-PCR analysis of circLMTK2 and LMTK2 mRNA after treatment with RNase R. (c) qRT-PCR analysis of circLMTK2 and LMTK2 mRNA after treatment with actinomycin D at the indicated time points. (d) qRT-PCR analysis of circLMTK2 and LMTK2 mRNA in either the cytoplasm or the nucleus. (e) RNA fluorescence in situ hybridization (FISH) for circLMTK2. The nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI). Scale bar, 5 μm