Fig. 3
From: circLMTK2 acts as a sponge of miR-150-5p and promotes proliferation and metastasis in gastric cancer
circLMTK2 promotes GC cell proliferation and tumourigenicity in vitro and in vivo. (a and b) Assessment of the proliferation of AGS and MGC-803 cells transfected with control or circLMTK2 siRNAs by CCK-8 assay. (c) Stable circLMTK2 overexpression promoted BGC-823 cell proliferation. OD, optical density. (d and e) Assessment of DNA synthesis using an EdU assay in MGC-803 and AGS cells transfected with control or circLMTK2 siRNAs. (f) Stable circLMTK2 overexpression promoted DNA synthesis in BGC-823 cells. Micrographs represent at least three experiments. Scale bar = 200 μm. (g and h) Colony formation assay using AGS and MGC-803 cells transfected with control or circLMTK2 siRNAs. (i) Stable circLMTK2 overexpression promoted BGC-823 cell colony formation. (j) Xenograft assay with BGC-823 stable cell lines. (k) circLMTK2 over-expression increased the volume of the xenograft tumours. (l) circLMTK2 over-expression increased the weight of the xenograft tumours. (a-l) Results are shown as the mean ± standard error of the mean (SEM) of three experiments. *P < 0.05; **P < 0.01; ***P < 0.001 (Student’s t-test)