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Fig. 5 | Molecular Cancer

Fig. 5

From: Mettl14 inhibits bladder TIC self-renewal and bladder tumorigenesis through N6-methyladenosine of Notch1

Fig. 5

Mettl14 overexpression inhibited bladder TIC self-renewal. a Mettl14 overexpressing cells were generated by lentivirus and confirmed by Western blot. oe, overexpression. b Mettl14 overexpressing cells were used for oncosphere formation assay. Spheres were shown in left panel and sphere-initiating ratios were shown in right panel. c Ki67 immunohistochemistry was performed using Mettl14 overexpressing and control cells. Typical images derived from patient #1 were shown. d Mettl14 overexpressing cells were used to detect CD44+ bladder TICs by FACS. The ratios of bladder TICs were shown. e, f Mettl14 overexpressing cells were used for transwell invasion assay. Typical images derived from patient #1 and invasive cell numbers were shown in E and F, respectively. g 5 × 106 Mettl14 overexpressing cells were utilized for tumor propagation and tumor weight was detected one month later. h, i 1 × 102, 1 × 103, 1 × 104 and 1 × 105 Mettl14 overexpressing cells were used for tumor initiation assay. Tumor formation ratios and bladder TIC ratios were shown in H and I, respectively. Six patients were all examined for tumor initiation assay, and only the results of patient #1 were shown. j, k Mettl14 overexpressing T24 cells were established, and used for sphere formation assay (j) and transwell invasion assay (k). l Mettl14 overexpressing T24 cells were used for tumor propagation assay, and the picture of established tumors was taken

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