Fig. 5
From: m6A modification suppresses ocular melanoma through modulating HINT2 mRNA translation
HINT2 acted as a tumor suppressor gene in ocular melanoma cells. a GSEA plots evaluating the changes in autophagy, mitochondrial function, apoptosis and cell death in ocular melanoma cells with or without HINT2 overexpression. NES, normalized enrichment score. b The animal experiment shows tumor suppressor roles of ALKBH5 and HINT2 in vivo. Top: The line graph shows the volume of subcutaneous tumors formed by the indicated OCM1 cells. Bottom: representative images of H&E staining to evaluate tumor formation. n = 5, **p < 0.01; scale bar: 5 mm. c, d HINT2 expression in normal and tumor tissues. C: HINT2 expression according to IF analysis. Scale bar: top panel, 100 μm; bottom panel, 20 μm. D: Statistical results of HINT2 expression in normal and tumor tissues. n = 105, ***p < 0.001. e Kaplan-Meier curves of tumor recurrence showing the difference between ocular melanoma patients with low and high HINT2 levels. n = 54, log rank test, p < 0.05. f The expression of HINT2 in patients at AJCC stages T1 to T4