Fig. 2

CircGSK3β promotes ESCC cell migration and invasion. a and b Scratch wound assays for circGSK3β depleted (a) or overexpressed (b) ESCC cells. The average sizes of the gaps were measured at the indicated times and expressed as mean ± S.D. of triplicates. c and d Transwell chamber assays for circGSK3β-depletion (c) or overexpressed (d) ESCC cells. The average numbers of migrated cells were counted after 24 h incubation and expressed as mean ± S.D. e and f Matrigel invasion assays for circGSK3β-depletion (e) or overexpressed (f) ESCC cells. The average numbers of migrated cells were counted after 24 h incubation and expressed as mean ± S.D. g and h EMT marker protein levels in ESCC cells with circGSK3β depletion (g) or overexpression (h) were detected by immunoblotting. i and j EMT marker protein levels in ESCC cells with circGSK3β depletion (i) or overexpression (j) were detected by Flow cytometry. *P < 0.05, **P < 0.01